The science behind T-NES

Our Products are made on a solid scientific research thanks to the kind cooperation of the University Of Rome Tor Vergata.

From the scientific literature we know that electromagnetic signals of a certain type significantly increase tolerance to hypoxia in post stroke patients, have strong angiogenetic properties and strongly reduce the cardiovascular risk factor^[5,6,7,8]

The scientific demonstrations of our biotechnology can be found below.

Improved endurance gain on mice by a nanotechnology device

Test su cavie

An official double-blind experiment involving a total of 300 mice was conducted in order to monitor running performance while monitoring cardiopulmonary function.

The Rotarod performance test is a test based on a rotating rod with forced motor
activity being applied. The test measures parameters such as riding time or
endurance of individual animals and is widely applied for testing the effect of
experimental drugs. Rotarod provides objective measures of balance, coordination, physical condition, motor-planning, and motor-coordination. The advantage of this test is that it creates a discretely measurable, continuous variable that can be used to quantify the effects of different conditions. This test is computerized and does not rely on subjective judgment by the operator.

Rotarod was performed inside a metabolism chamber which allowed measuring the percentage of maximal oxygen consumption (VO2 max), on top of the maximal heart rate (HR max), and calculating the aerobic threshold (maximal aerobic velocity, MAV) for each type of training.

Project Managers Prof. S. Beninati (University of Tor Vergata, Rome) and Prof.
Angelo De Martino (University of Tor Vergata, Rome).

Tipologie di allenamento eseguiti:

Types of training

Steady State Training (SST)
Low Intensity Steady State Training (LISST)
Steady State Split Session Training (SSSST)
High Intensity Endurance Training (HIET)

Groups:

Group A: Experimental, N=100 mices
Group B: Placebo, N=100 mices
Group C: No Device, N=100 mices

In groups A and B the devices were sutured to the mouse skin a short time prior to the Rotarod. All experiments were repeated three times independently. We observed no differences between groups B and C, thus the data from both groups was pooled together in the graphs below.

Finally, after all tests were performed, some Group A mice were picked randomly
and received further tests: computerized tomography of the heart, ecocardiogram,
blood analysis, and body muscle histology.

Results:

The results of this study were consistent with our previous expectations based on
pilot observations. Only mice in Group A showed a marked increase in maximal
oxygen consumption, and aerobic threshold, which were highest in the Steady State Training. Overall, the device achieves a 30-35% increase in global performance.

Importantly, no pathological findings were reported in any of these post-training
screenings performed on these animals.

Graphs:

Left bars: experimental Group A, N=100
Right bars: control Group B(PLACEBO DEVICE)+C (NO DEVICE), N=200

SST, Steady State Training

LISST, Low Intensity Steady State Training

SSSST, Steady State Split Session Training

HIET, High Intensity Endurance Training

Units:

VO2 Max as %
MAV as km/h

At least 100 observations± SD. Statistically significant (P< 0,05) increase of VO2 max (**) is reported in comparison to control (*)

The diagram shows the increase of VO2 max in Group A (left bars) in every types of training performed.

At least 100 observations± SD. Statistically significant (P< 0,05) increase of MAV max (**) is reported in comparison to control (*)

The diagram shows the increase of the MAV in the group A, which means that the Group A started to produce lactic acid at 20 km/h approximately, while Group B and C at 11-12km/h, in every types of training performed.

Istologia muscolare

Standard muscle 2 weeks after training with Placebo

T-NES System 2 weeks after training with LISST

T-NES System 2 weeks after training with HIET

Heart-Tomography after T-NES System, Group A:

Ecocardiogram after T-NES System, Group A:

Thanks to University of Tor Vergata, University of San Raffaele, Haybusak University, University of Catania and IRCSS S. Lucia, Rome. (S.Altobello et al. International Journal of Biomedical Materials Research, 2018)

Test on human athletes

The study was conducted on 60 male athletes (triathlon competitors) with an average age of 27.63 ± 1.54 years, an average weight of 77.23 ± 1.90 kg and a BMI factor of 23.73 ± 0, 91. The subjects played, after 3 min. of initial warm up, 6 total trials (3 trials with placebo and 3 with real deal), seven days apart from each other, of 10, 20, 30, 40 and 50 min. running at 9 km / h with an incremental gradient of 0.5% every 10 min., using a Technogym® Myrun treadmill (Technogym® SpA Via Calcinaro 2861 47521 Cesena, FC). The heart rate expressed in beats / min, has been determined by means of a Polar M460 HR Heart Rate Monitor (Polar Italy), while the serum lactate level, expressed in mmol / L, was evaluated using the LACTATE PRO 2 device (Arkray ™ Global Business.inc, Kyoto, Japan). The lactate and the HR were measured in the minute of slope increase. During the test period no athlete used the products, but the products were used only on the test days.

The heat rate values of the decreases obtained by the T-NES system, during the exercises, for the hearth rate, were:

7% – 10′
6,16% – 20′
6% – 30′
4,25% – 40′
7% – 50′

Only the exercise for 40’ (4.25%), has deviated from the 6.5% reduction average obtained for the other times.

The values of the decreases during the five exercises for serum lactate were:

27,63% – 10′
13,50% – 20′
11,80% – 30′
13,74% – 40′
12,65% – 50′

Only the exercise for 10’ (27.63%) has deviated from the 13% reduction average, obtained for the other times. In conclusion, the average reduction gained in both the parameters observed, thanks to T-NES system, was of about 6.5% for the HRmax and 13% for serum lactate levels compared to control.

(A. Johrian et al., Reduction by a Nanotechnology Device of Serum Lactate Levels and Heart Rate in Athletes Undergoing to Resistance Exercises, Journal of Biomaterials, 2018)

DATA OF PROFESSIONAL ATHLETES

Professional cyclist, tool used for surveys “Super Op”, in green the days when he used T-NES SYSTEM. FC= Heart rate.

Professional boxer, gloves sessions, 2 weeks with placebo (pic 1), 2 weeks later with T-NES SYSTEM (pic 2).

Professional boxer, heart rate under stress, with placebo.

Professional boxer, heart rate under stress with T-NES SYSTEM.

Test on a triathlete based on tapisroulant, running at 12km/h with incremental increase of 0,5 km/h every 5 min. right picture using T-NES SYSTEM, left picture with placebo.

Test on cyclist, bike, 150watt for 5 min, next 200watt for 5 min, and final peak. Right pic with T-NES, left with placebo.

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